Supplementary Data for Hua et al. (2020)

This dataset contains supplementary data for the analyses presented in:Zhengshuang Hua, Matthew Ouellette, Andrea M. Makkay, R. Thane Papke, and Olga Zhaxybayeva. Nutrient supplementation experiments on saltern microbial communities support utilization of DNA as a source of phosphorus (under review).Dataset contents:Quality_Control_script.zip: In-house Perl script used for quality control processing of raw Illumina reads. See README.md file inside the archive for details.merged_pairs.zip: Merged pairs from the rpoB and 16S rRNA data after the quality control step, removal of primers, and additional removal of low quality sequences. The sequences are in FASTA format.rpoB_database.zip: Curated database of 627 rpoB gene sequences used for taxonomic assignment of a representative sequence of an rpoB-based OTU. Halobacteria.rpoB.627.fa file contains nucleotide sequences in FASTA format. Halobacteria.rpoB.627.taxonomy file contains the taxonomic information for each sequence.OTU_tables_and_rep_seqs.zip: OTU tables for rpoB- and 16S rRNA-based OTUs (otu_table.rpoB.txt and otu_table.16S.txt files). Each row represents an OTU. Each column represent a sample. Values in the table show how many assembled contigs are in an OTU. Nucleotide sequences of chosen to represent each OTU are provided in rep_set_rpoB.fna and rep_set_16S.fna files, in FASTA format.