CIL:37067, Xenopus laevis, CNS neuron (sensu Vertebrata). In Cell Image Library

This is IMAGE #7 (time point: 8 hours)of an image group. It is a second z-stack of this neuron at this time point. It shows a neuron from the optic tectum of an albino Xenopus laevis tadpole CNS, stage 47. In the series, z-stacks of this neuron were acquired at a 2 hour interval for 8 hours. Afterward, a second time lapse series was acquired at a 60 second interval for 10 minutes. This time series shows dendritic arbor development and morphological plasticity of the dendritic arbor with branch additions and retractions. The cell was transfected with in vivo electroporation of plasmid DNA encoding eGFP ~24 hours before the image was acquired. The intact, living tadpole was anesthetized, positioned under a glass coverslip and this image was taken directly through its transparent skin using a custom built two-photon microscope