Analysis of gonadal fat from male control and adipocyte-specific LAMA4 knockout mice

To determine the transcriptomic effects of LAMA4 loss on adipose tissue structure and gene expression in two dietary paradigms, we assessed gonadal white adipose tissue of male control and adipocyte-specific LAMA4 knockout mice using a Quant-Seq strategy. Epididymal fat pads were dissected from a) 6 male wild-type and 6 male adipocyte-specific LAMA4 knockout mice following 20 weeks of regular diet feeding, and b) 6 male wild-type and 6 male adipocyte-specific LAMA4 knockout mice following 20 weeks of 45% fat diet feeding, followed by isolation of total RNA and messenger RNA quantification via Quant-Seq.