Transcriptome sequencing of aerial bulb in garlic

The long-time asexual reproduction of garlic with garlic cloves has resulted in virus accumulation and genetic depression. The seedling propagation of garlic by means of tissue culture can both eliminate viruses and improve breeding efficiency. Aerial bulbs are the first-choice materials for virus-free seed-breading of garlic under external condition, but aerial bulbs are featured by dormancy just like garlic bulbs, and low temperature or GA3 can quickly break dormancy. This research is designed to use high-throughput sequencing method to perform the sequencing research of dormant aerial bulbs and those dormancy breaking; to screen out the key differential expression genes of aerial bulbs corresponding to low temperature or GA3; and to provide a theoretical basis for exploring the molecular mechanism for dormancy breaking of aerial bulbs.