Isolation and characterization of human monoclonal antibodies to digoxin.
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Epitope-specific mAbs 7F2-31, 11E6-7, and 5C2-4 had about a 4.5- to 5.5-fold lower affinity for digoxigenin than for the epitope. The Ki values of mAbs 7F2-31, 11E6-7, 5C2-4 for digoxigenin were 7.2, 27, and 140 nM, respectively, as determined by competitive radioligand binding to [3H]digoxin.
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1999-01-01



