Real-time qRT-PCR analysis in RNA pools derived from BPH and prostate cancer.

Comparison between gene status in BPH and prostate cancer. BPH categories are mild, moderate (Mod), and severe (Sev) and prostate cancer (CaP) pools are derived from both moderately and poorly differentiated tissues. Average expression levels 6-fold were defined as “high”, and levels between 1-fold and 6-fold were defined as “moderate”. Expression levels are relative to a control pool derived from TZ samples from patients with no appreciable TZ expansion. Genes that appear twice were measured using two distinct primer sets. BPH/CaP is the ratio of the average levels in the BPH pools divided by the average levels in the prostate cancer pools.