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mRNA isoform detection by long-read sequencing of 10x single cell cDNA libraries

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NIAID Data Ecosystem2026-05-02 收录
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https://www.ncbi.nlm.nih.gov/sra/ERP168734
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In this study, cells from K562 human cell line were prepared using Chromium (10X Genomics) scRNA-seq method that was modified to yield full length cDNA libraries to be sequenced on a PromethION instrument (Oxford Nanopore Technologies) using chemistries R10.4.1 and R9.4.1. Three different methods for obtaining full length were tested: FLT-seq (Tian et al. (2021)), SQK-PCS111 (ONT) and EXP-PCA001 (ONT).
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2025-02-02
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