Uncovering the transcriptional changes in egressed vs intratumoral effector CD8+ T cells

Comparative gene expression profiling of RNA-seq data for tumor-retained, tumor-egressed, and circulating effector CD44+ CD8+ T cells. Overall design: YUMMER1.7 tumors were implanted into Kaede-Tg mice. On day 11, tumors were photoconverted, and on day 12, YUMMER1.7 tumors, draining lymph nodes, and spleens were harvested. Kaede red+ CD44+ CD8+ T cells were sorted from YUMMER1.7 tumors (tumor-retained) and draining lymph nodes (tumor-egressed) along with CD44+ CD8+ T cells from the spleens (circulating effector CD8+ T cells). Tissues were harvested and sorted from 4 replicate mice. MedGenome Inc performed RNA extraction, library prep, and sequencing.