Hirschmanniella mucronata rDNA sequences from Cambodian populations
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In Cambodia, 79 plant-parasitic nematodes were isolated from rice roots in 2014 and 2015 (12 ° 32'55.6 \"N 105 ° 08'48.6\" E). The gDNA was extracted and the PCR was performed with Internal Transcribed Spacer (ITS) primers rDNA2 (5'-TTGATTACGTCCCTGCCCTTT-3 ') and rDNA1,58s (5'-ACGAGCCCGAGTGATCCACCG-3') (Vrain et al., 1992, Powers 2004, Pokharel et al ., 2007). The PCR products were sequenced using Sanger Sequencing primers (rDNA) and all chromatograms are deposited in the following document.
创建时间:
2023-11-22



