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RNA-seq study of directed sclerotome, Lon non-coding RNA PEAT, and the embryonic Dll1 lineage form E9.0-9.5

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We sequenced RNA from embryonic mouse tissue directed to form sclerotome in culture. Using Crispr/Cas9, we generated a mouse line bearing a complete deletion of the PEAT, a LncRNA adjacent to Pax1 and highly enriched in sclerotome. We perform RNA-seq on samples from 4 stages of maturation of the Dll1 lineage. Examination of sclerotome using RNA-seq

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