Single-cell sequencing of E12.5 mouse placenta

In our study, we performed single-cell sequencing of the E12.5 mouse placenta to explore the cellular diversity and gene expression dynamics during placental development. This approach allowed us to delineate the complex landscape of cell types and states present at this critical stage of gestation. By analyzing the transcriptomic data, we identified several distinct trophoblast subpopulations, each characterized by unique gene expression patterns that suggest different functional roles in placental structure and function. Additionally, our data provided insights into the potential regulatory pathways involved in cell differentiation. Overall design: We utilize placental samples from E12.5 mice, specifically choosing two distinct genotypes: wild-type (control group) and Elf5-Cre; Slc25a1f/f (experimental group). Wild-type placentas serve as control samples to evaluate the impact of Slc25a1 deletion on placental development.