Head-to-Head CRISPRko CRISPRi Perturb-seq: Aligned, filtered data with guide assignments
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Low MOI perturb-seq data (as Seurat objects) from parallel Cas9 CRISPRko and CRISPRi benchmark libraries. Fastqs were aligned with Illumina DRAGEN software, which filters droplets to "passing cells". Cells are further filtered to those with <15% mitochondrial transcripts and a single guide assigned. Guide assignments are stored in the metadata of each Seurat object. Since CRISPRko and CRISPRi are sequenced separately at Day 14, the distinct Rds for each modality is provided to distinguish negative control cells perturbed with CRISPRko or CRISPRi. See version 1 for all Rds files.
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Zenodo创建时间:
2026-07-09



