The landscape of small nucleolar RNA expression in multiple myeloma is determined by cytogenetic alterations (RibomethSeq)

We combined mRNA, small RNA and ribosome 2'O-methylation sequencing to investigate snoRNA expression patterns in multiple myeloma and their association with different chromosomal aberrations. We identified snoRNAs dysregulated in molecular subgroups, with SNORD78 being highly expressed in gain1q patients and associated with poor prognosis. Our study shows that the expression of particular snoRNAs and methylation of specific snoRNA-guided rRNA sites are associated with specific chromosome gains, which are common elements in multiple myeloma. Overall design: We performed RiboMethSeq to measure 2'O-methylation of ribosomal RNA in 26 newly diagnosed multiple myeloma patient samples.