Expression analysis of microRNAs in plasma of new HIV-infected cases, old HIV-infected cases and healthy controls.

There are 19 differentially expressed microRNAs among new HIV-infected cases, old HIV-infected cases and healthy controls. Five microRNAs show trends in healthy controls, new HIV-infected cases and old HIV-infected cases, they are hsa-miR-1291, and hsa-miR-3609 with up-trends, and hsa-miR-3162-3p, hsa-miR-874-5p and hsa-miR-4258 with down-trends. A microarray study using total RNA extracted from the plasma of ten new HIV-infected cases (estimated duration of infection <1 year), ten old HIV-infected cases (estimated duration of infection≥ 1 year) and ten healthy controls. Each array measures the expression level of 3,100 microRNAs annotated in miRBase 18.0, covering all human, mouse and rat, as well as all virus microRNAs related to these species. In addition, this array contains capture probes for 25 miRPlus™ human microRNAs.