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Proteomic identification of Amuc_1100-interacting proteins by GST pull-down LC–MS/MS

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To identify potential interacting proteins of the outer membrane protein Amuc_1100 from Akkermansia muciniphila, GST pull-down assays were performed using lysates from HTR-8/SVneo cells, mouse placenta, and small intestine tissues. The bound proteins were analyzed by LC–MS/MS on an Orbitrap Exploris 480 mass spectrometer

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2025-11-13
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