Transcriptome profiling of Pseudomonas syringae pv. syringae 9644 in rich media and hrp-inducing minimum media

Pseudomonas syringae pv. syringae 9644 (Pss9644) is a causal agent of bacterial cherry canker causing necrotic symptoms on leaves, fruits, gummosis and canker in woody tissues of sweet cherry (Prunus avium). To understand which virulent factor genes were expressed in vitro, Pss9644 was grown in rich media (King's B Broth) and minimum media (hrp-inducing minimum media). The latter mimics the in planta environment. Pss9644 was grown in King's B broth overnight. Later, it was diluted (OD= 0.1) until grown to log phase (OD=0.6). Cells were washed and shaking incubated in King's B broth and hrp-inducing minimum media Cells were harvested after 5 hours post incubation. Total RNA was extracted. RNA samples underwent ribosomal depletion before sequencing.