PP2Aca regulates cerebellar development

The raw data of our manuscript "PP2Aca regulates cerebellar development via phosphorylation-dependent neural programs" are shown here.
All the raw data has been organized and sorted according to the order of the figures in the article.
Here is the abstract of the manuscript:
The development of the cerebellum requires precise control of phosphorylation, yet the contribution of protein phosphatases remains poorly understood. Here we investigate the function of protein phosphatase 2A catalytic subunit (PP2Acα) in cerebellar granule neuron. Conditional deletion of Ppp2ca using Atoh1-Cre mice resulted in impaired motor coordination and cognitive performance, indicating disrupted cerebellar development. Histological analyses revealed abnormal granule neuron proliferation, migration and defective Purkinje cell organization during early postnatal stages. Phosphoproteomic and proteomic profiling of primary granule neurons revealed PP2Acα-dependent regulation of cell cycle control, cytoskeletal remodeling, RNA splicing, metabolism, and translation. Extending these analyses to whole cerebellar tissues uncovered broader system-level changes affecting neuronal morphogenesis, mRNA processing, synaptic signaling, and behavior. Collectively, these findings establish PP2Acα as a critical regulator of cerebellar development through multilayered control of phosphorylation and protein homeostasis, providing mechanistic insights into phosphatase-related neurodevelopmental disorders.