Levels and distributions of the Sec tRNA[Ser]Sec isoforms in liver of micea.

aBulk tRNA was isolated from liver of the four mouse lines (column 1) that were maintained on different diets (column 2), the tRNA aminoacylated with 3H-serine, the resulting labeled seryl-tRNA fractionated on a RPC-5 column, the percent of the i6A−and wild type seryl-tRNA[Ser]Sec isoforms within the total seryl-tRNA[Ser]Sec population determined (columns 3 and 4, respectively), the distributions of mcm5U and mcm5Um assessed as the percent of wild type mcm5U and mcm5Um seryl-tRNA[Ser]Sec isoforms within the total seryl-tRNA population (columns 5 and 6, respectively) and ratio of mcm5Um to mcm5U determined as described in Materials and Methods.