Matched primary ovarian cancer, omental metastases, and non-omental metastases from 10 patients with high-grade serous ovarian carcinoma

Matched high-grade serous ovarian carcinoma samples collected from the ovary (ov), omental metastasis (om-met), and non-omental intraperitoneal metastasis (met) from 10 patients at the time of primary debulking surgery were analyzed for RNA expression by RNA sequencing. Overall design: FFPE blocks of matched high-grade serous ovarian carcinoma samples collected from the ovary (ov), omental metastasis (om-met), and non-omental intraperitoneal metastasis (met) from 10 patients were sectioned onto uncharged glass slides. One 4 µm H&E-stained section was used by a pathologist to circle the tumor areas and delineate them from the adjacent normal tissue. Depending on the tumor size, 1 to 3 unstained 10 µm sections were macrodissected (removal of non-tumor areas based on the H&E template) with a clean razor blade. Total RNA was isolated using the miRNeasy FFPE kit. RNA sequencing was done by MedGenome.