Gene expression of mouse small intestinal 3D organoid myofibroblast-epithelium co-culture

The gene expression of mouse wt small intestinal epithelium and myofibroblast,3D organoids, both in monoculture and in co-culture, as well as small interstinal adenoma organoids from the Apc+/1638N mouse model, were measured by Affymetrix Mouse Gene 2.1 ST Array. Mice were sacrifced at 8-10 weeks Gene expression of the following mouse small intestinal cells were analyzed a. wt epithelium cells in monoculture (n=2) b. wt epithelium cells in co-culture (n=2) c. Apc+/1638N adenoma cells (n=4 mice, 6 samples) d. wt fibroblasts in monoculture (n=3) e. wt fibroblasts in co-culture (n=3)