Single cell RNAseq analysis of Igbpl1 treatment on microbead-induced glaucoma in mice

IGFBPL1 deficiency leads to progressive retinal neuron loss and phosphorylated Tau accumulation in the brain. To investigate the role of IGFBL1 in retina microglia homestasis and function. Total immune cells were sorted from microbead (MB)-induced glaucoma mice and glucoma mice treated with recombinant Igfbpl1 protein for single cell RNAseq analysis by 10X genomics technology. Overall design: scRNA-seq was performed for immune cells isolated from retinas of 10 MB and 10 Igfbpl1-treated MB mice (at 8-10 months of age), using FACS. Mice were transcardially perfused with saline, and digested into single cell suspension in a dissociation solution that included collagenase/dispase (1 mg/ml), papain (80 units), and Dnase (0.5mg/ml) in DMEM for 1 hour at 37oC.