Global pleiotropic effects in adaptively evolved Escherichia coli lacking CRP reveal molecular mechanisms that define growth physiology. Global pleiotropic effects in adaptively evolved Escherichia coli lacking CRP reveal molecular mechanisms that define growth physiology

We report high-throughput RNA sequencing of Δcrp and five adaptively evolved mutants of Δcrp in the batch exponential phase, IG116-Δcrp in batch exponential phase and Δcrp and two adaptively evolved mutants of Δcrp in chemostat cultivations Overall design: RNA was extracted from two biological replicates of WT, Δcrp , five adaptively evolved mutants of Δcrp and IG116- Δcrp in the batch exponential phase grown in glucose minimal media conditions. For glucose-limited chemostat cultivations, RNA was extracted from two biological replicates of WT, Δcrp , two adaptively evolved mutants of Δcrp after 3-5 residence times at steady state. RNA sequencing was done using Illumina Hi-Seq 2500 (Rapid Mode) platform.