Materials used in the mutagenesis process for creating plasmids with two Lac repressor binding sites.

Primer sequences(5���3��):Mut0: ctaactcacattaattgcgttgAgctcGAGgTTcgctttccagtc.Mut1: catacgagccggaa (G) cataaagtgtaaagc.Mut2: ctcggaaagaaca AATTGTGAGCGCTCACAATT aaggccaggaacc.Mut3: ctcggaaagaacat AATTGTGAGCGCTCACAATT aggccaggaaccg.Mut4: cggaaagaacatg AATTGTGAGCGCTCACAATT ggccaggaaccgt.Mut5: ggaaagaacatgt AATTGTGAGCGCTCACAATT gccaggaaccgta.Mut6: gaaagaacatgtg AATTGTGAGCGCTCACAATT ccaggaaccgtaa.Mut7: cggaaagaacatgtga AATTGTGAGCGCTCACAATT caggaaccgtaaaaag.Mut8: ggaaagaacatgtgag AATTGTGAGCGCTCACAATT aggaaccgtaaaaagg.Mut9: gaaagaacatgtgagc AATTGTGAGCGCTCACAATT ggaaccgtaaaaaggc.Mut10: catacgagccggaag [C] cataaagtgtaaagc.Mut11: catacgagccggaag [CG] cataaagtgtaaagc.The capital letters in the primer sequences indicate the mutations. ��()�� indicates bp deletion and ��[ ]�� indicates bp addition. The inter-operator distance indicated here is the distance between two inner edges of the operators instead of center to center distance that is commonly used in in vivo experiments [11]�C[13], [86], [93].