MFold Program was used to predict the stability of the secondary structure of TS:N20 RNA and the mutant constructs. The nucleotides in bold represent point mutations.
All values are given as percentages. Consensus structures were generated by RNAalifold [21] using SHAPE-directed sequence alignments (column pair 4). These structures were then used to constrain indiv
The center column shows the recovery rate across our predictions and the right column the recovery rate across all of our annotated structures, including those which did not meet our discovery thresho