Non-transmissible measles virus vector with segmented RNA genome establishes different types of iPSCs from hematopoietic cells

We established iPSCs from CD34+ HPCs or CD3+ T cells by transducing measles virus vectors encording reprogramming genes. HPC-derived iPSCs and T-cell-derived iPSCs showed naive-like and primed pluripotent cell properties, respectively. Overall design: CD34+ and CD3+ cells were sorted from cord blood and transduced with measles virus vectors encoding OCT3/4, SOX2, KLF4 and L-MYC. The cells were cultured in regular human ESC culture media for 2-3 weeks. After development of colonies, the cells were harvested and dissociated, then cultured with in naive media or human ESC culture media. After several passages, total cell RNA was harvested and subjected to RNA seq.