RNA-seq of gastrocnemius (GA) muscle tissue from mice treated with extracellular vesicles (EV) from MCF-10A or MDA-MB-231 cells

To compare gene expression changes in skeletal muscle caused by EVs from normal (MCF-10A) and cancer (MDA-MB-231) cells, we analyzed RNA isolated from the GA muscle of EV-treated female NOD scid gamma (NSG) mice. Mice had received tail-vein injections of EVs twice a week for 5 weeks (~10 ug EV per injection). Gene expression in muscle from mice treated with MDA-MB-231-derived EVs was compared to mice treated with MCF-10A-derived EVs. Overall design: RNA-seq was used for transcriptomic profiling of GA muscle following a total of 10 times of intravenous EV injections (MCF-10A EVs or MDA-MB-231 EVs) in NSG mice. RNA-seq and data processing were performed by Novogene Co., Ltd.