2019-05-05-modularized-crispr-dcas9-effector-toolkit.pdf
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We generated a nuclease-deficient dcas9 sequence (based on ref1). The human influenza hemagglutinin derived HA-tag and anuclear localization sequence (NLS) derived from the adeno associated virus serotype 2 (AAV2-NLS) were fused N terminally to dCas9 via BioBrick standard assembly (Figure1a). A human cytomegalovirus promoter (hCMV) was used todrive expression of the constructs in mammalian cells. We fusedeffector domains C-terminally to dCas9 to achieve transcrip tional regulation of genes of interest.
创建时间：
2019-05-05
