Features of several “NLSs” from ASR1 homologues.

NLSs of ASR homologues from different species were compared to that of tomato ASR1. NUCLEO software [20] renders high probability of nuclear localization (“predicted NLS” column) for most of the polypeptides. Similarly, YLOC [19] predicts high probability and high confidence of nuclear localization for all the orthologous proteins except for citrus. Species with experimentally proven nuclear localization for ASR are indicated in the “observed” column. Alignment of all NLSs reveals differences in the carboxy-terminal region at the lysine (K) positions highlighted in gray. Molecular weight (MW) of full length mature polypeptides is shown.