The manufacturing method of polyisoprenoid， the manufacturing method of a vector， a transformed plant， and a pneumatic tire， and the manufacturing method of rubber goods

The present invention provides a method that is for producing a polyisoprenoid， that augments the rubber-synthesis activity of rubber particles， and that makes it possible to increase production of natural rubber. The present invention is a method that is for producing a polyisoprenoid in vitro， that uses a gene that codes for a protein in the cis-prenyltransferase (CPT) family and a gene that codes for a protein in the Nogo-B receptor (NgBR) family， and that uses rubber particles to which are bound the proteins for which the genes code. In the method for producing the isoprenoid， a vector wherein a gene that codes for a protein in the CPT family and a gene that codes for a protein in the NgBR family have been bound to a promoter that promotes latex-vessel-specific gene expression is introduced into a plant and latex-vessel-specific expression of the proteins for which the genes code is induced. The present invention provides a method that is for producing a polyisoprenoid， that augments the rubber-synthesis activity of rubber particles， and that makes it possible to increase production of natural rubber. The present invention is a method that is for producing a polyisoprenoid in vitro， that uses a gene that codes for a protein in the cis-prenyltransferase (CPT) family and a gene that codes for a protein in the Nogo-B receptor (NgBR) family， and that uses rubber particles to which are bound the proteins for which the genes code. In the method for producing the isoprenoid， a vector wherein a gene that codes for a protein in the CPT family and a gene that codes for a protein in the NgBR family have been bound to a promoter that promotes latex-vessel-specific gene expression is introduced into a plant and latex-vessel-specific expression of the proteins for which the genes code is induced.