The pancreatic cancer stem cell marker CD44v6 affects transcription, translation and signaling: consequences on exosome composition and delivery

The comparison between the impact of claudin7 and palmitoylation site mutated claudin7 on miRNA and proteins in cells versus exosomes allowed to judge on linked versus independent recruitment of miRNA and proteins and the impact of claudin7 on the recruitment of the mRNA processing machinery into exosomes.通过对比紧密连接蛋白7（claudin7）与棕榈酰化位点突变型claudin7分别对细胞及外泌体（exosomes）内微小RNA（miRNA）和蛋白质的影响，可判断微小RNA与蛋白质的募集模式为相互关联还是彼此独立，并阐明紧密连接蛋白7对mRNA加工复合体（mRNA processing machinery）向外泌体中募集的作用。