Transcriptome of c-kit+ cells in rat under stress

RNA-sequencing analysis of the c-Kit positive cells under stress to identify novel factors or pathways involved in rat stress erythropoiesis. Overall design: 4 female and 2 male 6-8 week old ACI rats were randomly divided into 2 group, each group has 2 females and 1 male. 100mg/kg PHZ or equal amount of PBS were injected through the peritoneal route. The rats were sacrificed on 36hr after injection and total bone marrow cells were isolated from hind legs of ACI rats. Single cell suspension was made and incubated with biotin-c-kit antibody for 20min on ice. Then the cells were washed with PBS and suspended with streptavidin particles followed by incubation on ice for an additional 20 minutes. C-kit positive cells were purified using EasySep column free cell isolation system. Then total RNAs were extracted using TRIzol and processed to RNAseq.