Genome-wide Maps of Chromatin State in Control and DEK-deficient Hematopoietic Stem Cells [CUT&Tag]
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE166433
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Purpose:To identify chromatin state involved in the DEK regulating gene expression of hematopoietic stem cells (HSC) in mice, we performed DEK, H3K27ac, H3K4me3 and H3K9ac CUT&Tag in HSC or LSK cells freshly sorted from mice. Methods: About 20,000 HSC (Lin-Sca-1+c-Kit+CD48-CD150+) or 50,000 LSK cells (Lin-Sca-1+c-Kit+) in bone marrow were isolated by FACS from Dekfl/fl (floxp) and Dekfl/fl,Tie2-Cre (cKO). Genomic DNA profiles of HSC or LSK cells treated with DEK, H3K27ac,H3K9ac, and H3K4me3 antibody were generated by deep sequencing, using Illumina HiSeq 2000.
创建时间：
2021-05-24
