Reaumuria songarica Raw sequence reads

Differential transcriptome analysis and identification of Reaumuria songonica leaves were performed with some modifications. Briefly, total RNA was extracted from the collected samples using the plant RNA purification kit (Norgen, Thorold, Ontario, Canada) according to the manufacturer's instructions. The quality of total RNA was then measured using an biological analyzer Agilent 2100 Bioanalyzer (Agilent, Santa Clara, California, America). Oligo (dT) magnetic beads (Biomag, Wuxi, Jiangsu, China) were used to enrich the mRNA with PolyA structure in total RNA, and the mRNA was cleaved into 200-300 bp fragments by ion disruption. Using the RNA as a template, the first strand of cDNA was synthesized using 6-base random primers (Gdsbio, Guanzhou, Guangdong, China) and reverse transcriptase (Aidlab, Haidian, Beijing, China). The first strand cDNA was used as a template to synthesize the second strand cDNA, and the library size was 300-400 bp. Quality control was performed by Agilent 2100 Bioanalyzer and double-terminal sequencing was performed by Illumina HiSeqTM2000 (NGS Solexa Hiseq2000, Illumina, California, America).