Dynamic maps of protein-protein-metabolite complexes of Arabidopsis Thaliana seedlings

The project aimed to create dynamic maps of protein-protein-metabolite complexes of Arabidopsis thaliana seedlings using PROMIS (PROtein–Metabolite Interactions using Size separation). The approach involves using size exclusion chromatography (SEC) to separate complexes, followed by LC-MS-based proteomics and metabolomics analysis of the obtained fractions. Co-elution is used to reconstruct the protein-metabolite interactions (PMIs) networks. PROMIS strongly progresses understanding protein-small molecule interactions due to its non-targeted manner, cell-wide scale, and generic nature, making it suitable across biological systems. Combining PROMIS with mashing learning approach SLIMP “supervised learning of metabolite-protein interactions from multiple co-fractionation mass spectrometry datasets” allows computing a global map of metabolite-protein interactions in vivo.