miRNA transcriptome profiling of Chlorella sorokiniana and Chlorella zofingiensis under normal and stress condition

Purpose: The purpose of this study are to identify the miRNA involved in regulating the production of metabolites in Chlorella sorokiniana and Chlorella zofingiensis under normal and stress-induced condition through RNA-sequencing technique. Methods: miRNA transcriptome profile from normal and stress sample of C. sorokiniana and C. zofingiensis were generated, in triplicate, using Illumina Miseq. The sequence reads that passed quality filters were analysed using CLC genomic workbench and OmiRas. Results: The known and predicted novel miRNAs were identified. Although most of the identified miRNAs were not functionally determined, this study suggests that they were species-specific, which may have roles in regulating genes during stress related condition. miRNA transcriptome profile of C. sorokiniana and C. zofingiensis under normal and stress conditions were generated in triplicates by deep sequencing using NEBNext Multiplex Small RNA Library Prep for Illumina.