Single cell RNAseq in HIV/HCV co-infected individuals with drug use

The US is experiencing a major drug epidemic largely attributed to synthetic opioids including fentanyl. Whole blood was collected from adult patients with HIV experiencing overdose at the University of Cincinnati Medical Center, as well as non-opioid using controls. Single cell barcoding and complementary DNA were performed using Chromium Next GEM Single Cell 5' Reagent Kits v2. These findings suggest multiple pathways by which opioid use contributes to HIV pathogenesis.