RNA-seq analyses of primary human fetal RPE cells (hfRPE) spanning six months in culture

In this study, we used an in vitro RPE model mimicking extracellular deposit formation and atrophy to study age-related changes occurring during early-AMD pathogenesis. Analysis of gene expression profiles at 4, 12, 17, and 25 weeks of culture revealed significant alterations in the RPE transcriptome related to age. The results of this analysis, together with those obtained through other omics such as metabolomics, are relevant to improve knowledge about RPE aging and the pathophysiology of AMD.