Deletion hotspots of AMACR CGI and their relation to the level of AMACR expression and colon histological entity.

Samples were divided into five groups according to their AMACR expression score (Left) or into six groups according to their histologic entity (Right). Left: Deletion at CG3 (��3 only) fluctuated as AMACR expression level went from 0 to 4; deletion at CG10 (��10 only) was a rare (��5%) event in colon cells; Notably, CG3 and 10 double-deletions (��3 & 10, bold) were the only deletions identified in groups with low AMACR expression (score: 0�C1). Frequent CG12-16 deletion (��12-16 only, bold) was correlated with high AMACR expression (53%, 67% and 66%, respectively. Score: 2�C4). The rest of deletion combinations (��3 & 12-16; ��10 & 12-16; and ��3, 10 & 12-16) were not found or were at a low level (*, p