Microbiome shifts in Pacific oysters (Magallana gigas) under nutritional stress (23C, starved), 16S rRNA metabarcoding nutritional stressed samples, DECICOMP project

Nutritional stress can influence the microbiome composition of Pacific oysters (Magallana gigas), potentially impacting disease resistance. This study investigates the microbial communities of two families (F11N and F14R) of 3-month-old disease-susceptible from biparental oysters, maintained under permissive conditions (23C) but deprived of food (starved condition). This acclimatation lasted 16 days before infection via cohabitation for 72 hours with sampling at 0h, 3h, 6h, 12h, 24h, 48h, and 72h. Each sample is a pool of 10 oysters, with three replicates. DNA was extracted, and 16S rRNA metabarcoding sequencing (V3-V4 regions) was performed using an Illumina MiSeq platform (2x250bp). The dataset provides insights into the impact of food deprivation on oyster-associated microbiota.