Triple therapy reduced metastasis in triple-negative breast cancer.

Purpose: To understand the underlying mechanisms of oncolytic virus therapy in breast cancer. Methods: The primary tumors of 4T1 in BALB/C mice were exstracted and analyzed by RNA-seq. Results: In consistent with previous studies, we found some anti-tumor factors were up-regulated. Interestingly, several immunosupressive genes were activited. Conclusion: Our study identified the intercellular and intracellular factors restricting the optimized oHSV efficacy and supported the rationally designed triple therapy for clinical translation. Breast tumors in BALB/C mice were extracted, followed by RNA-seq. There were 4 groups, and 4 mice per group. Control group: Intratumoral and intraperitoneal injection of PBS. Ab group: Intraperitoneal injection of PD-1 antibody. Dual group: Intratumoral injection of oHSV and intraperitoneal injection of OLA. Triplet group: Intratumoral injection of oHSV and intraperitoneal injection of OLA and PD-1 antibody.