Tree forms of different genes with best fitting nucleotide substitution model under the Bayesian framework using MrBayes.

The labels for the taxa used in the tree forms are 1 = Amphiprion bicinctus, 2 = A. polymnus, 3 = A. frenatus, 4 = A. perideraion, 5 = A. clarkii, 6 = A. ocellaris, 7 = A. percula, 8 = Premnas biaculeatus, 9 = Abudefduf vaigiensis and 10 = Chaetodon auripes. The tree forms can be classified four types based on the position of P. biaculeatus, which are type I (({[1~5][(6,7)8]}9)10) (i.e. the percula complex and P. biaculeatus were grouped into one clade, which formed the ancestral taxon of the anemonefishes), type II (({[8][1~5][6,7]}9)10) (i.e. the basal group of anemonefishes was the percula complex, while the P. biaculeatus was at the end of Amphiprioninae), type III (({[(1~5][8][6,7]}9)10) (i.e. the basal group of anemonefishes was the percula complex, while the P. biaculeatus was in the middle of Amphiprioninae) and type IV (({[(1~5)8][6,7]}9)10) (i.e. the basal group of anemonefishes was the percula complex, while the P. biaculeatus was the root of another clade grouped with subgenera Amphiprion, Paramphiprion and Phalerebus).—, not applied.
Tree forms of different genes with best fitting nucleotide substitution model under the Bayesian framework using MrBayes.