Table_1_Identification of Five Key Genes Involved in Intrinsic Apoptotic Pathway From Yellow Catfish Pelteobagrus fulvidraco and Their Transcriptional Responses to High Fat Diet (HFD).DOC

The hypothesis of the present study is that apoptosis through an intrinsic mitochondrial pathway may mediate high fat diet (HFD)-induced changes in the metabolism of Pelteobagrus fulvidraco. To this end, we cloned the full-length cDNA sequences of Cycs, Apaf1, Casp9, Casp3a, and Casp3b involved in the mitochondria apoptotic pathway, and explored their mRNA tissue expressions and transcriptional responses to HFD. All of these members shared similar domains to their orthologous vertebrate genes. They were constitutively expressed in all analyzed tissues but varied from tissue to tissue. Compared to the control, HFD up-regulated the mRNA expression of partial genes among these five key genes (Cycs, Apaf1, Casp9, Casp3a, and Casp3b) in mesenteric fat, intestine, ovary and the kidney, indicating the induction of apoptosis in these tissues; in contrast, HFD down-regulated mRNA levels of partial genes among the five key genes (Cycs, Apaf1, Casp9, Casp3a, and Casp3b) in the heart, spleen and gill tissues, indicating the inhibition of apoptosis in these tissues. The present study will facilitate further exploration into the functions of these genes at the molecular level and disclose the critical involvement of these genes against nutrient changes, indicating that processes of apoptosis in various tissues may differentially be modified by HFD.

本研究提出之假设为：通过线粒体内在途径介导的细胞凋亡可能调节高脂肪饮食（HFD）对黄颡鱼（Pelteobagrus fulvidraco）代谢的影响。为此，我们克隆了参与线粒体凋亡途径的Cycs、Apaf1、Casp9、Casp3a和Casp3b全长cDNA序列，并探讨了它们在HFD作用下的mRNA组织表达和转录反应。这些成员与其同源脊椎动物基因具有相似的结构域，在所有分析组织中均呈组成性表达，但表达水平在不同组织中存在差异。与对照相比，HFD在肠系膜脂肪、肠道、卵巢和肾脏中上调了这五个关键基因（Cycs、Apaf1、Casp9、Casp3a和Casp3b）的部分基因的mRNA表达，表明这些组织中细胞凋亡的诱导；相反，HFD在心脏、脾脏和鳃组织中下调了这五个关键基因的部分基因的mRNA水平，表明这些组织中细胞凋亡的抑制。本研究将有助于进一步探索这些基因在分子层面的功能，并揭示这些基因在对抗营养变化中的关键作用，表明HFD可能通过不同组织中的细胞凋亡过程的不同调节来影响这些基因。