Characterizations of pre-master (P7) seeds.

aCloned viruses (by serial plaque purifications) selected for further development of MVS are designated in bold. b*: Reversion rate <0.07% (detection limit). **: Reversion rate <0.01% (detection limit). cPlaque phenotypes: P2: similar to P2 virus; L = larger than P2 virus, D = similar size, but appear somewhat different in clearness of the plaques; S = smaller than P2. dSubstitutions differing from the engineered DENVax cDNA clones. Amino acid mutations are listed with residue position in the viral proteins. Total numbers of silent mutation in structural and non-structural genes, as well as substitution at non-coding regions (5′ or 3′NCR) are also noted. end = Not done. These clones had higher 5′NCR-57 reversion rates (by TaqMAMA) than other clones, so were excluded from further sequence analysis.