Phylogenetic reconstruction and species distribution data for C. reinhardtii VIA1 (Cre07.g338350)

Study Context & Key FindingsThis dataset contains supplementary phylogenetic data supporting the analysis of the VIA1 protein in C. reinhardtii (Cre07.g338350). These data were generated to validate and extend the GreenCut assignment of VIA1 by reconstructing its phylogeny and assessing its distribution across eukaryotic and prokaryotic lineages. The analyses supported by these files demonstrate that VIA1 is highly conserved across most photosynthetic eukaryotes, including species bearing primary and secondary plastids, though it has been frequently lost in dinoflagellates. The presence of homologs in cyanobacteria, alongside the eukaryotic distribution, supports a plastid-associated origin for VIA1 dating back to the primary cyanobacterial endosymbiosis in the Viridiplantae.MethodologyTo reconstruct the VIA1 phylogeny, the C. reinhardtii ortholog (Cre07.g338350) was searched against a series of predicted proteomes representing eukaryotic and prokaryotic diversity using Diamond BLASTP v2.1.11 (ultra-sensitive, E This item includes protein sequences, alignments, phylogenies, and datasets used to perform this analysis.