Antibody production and brain leukocyte infiltration at day 45 post- infection.

A–B. Sera were collected from non-infected (NF) or mice which were intracranially (i. c.) infected with WNV H8912 at day 45 (D45) post-infection. The development of WNV-specific IgM (A) or IgG (B) antibodies was determined. C–F. Levels of CD4+ T cell (C), CD8+ T cell (D), monocyte (CD11b, E) and neutrophil (Ly6G, F) expression in the three WNV-infected mice brains were measured by Q-PCR assay at day 45 post-infection. The y-axis depicts the ratio of the amplified CD4, CD8, CD11b or Ly6G cDNA to β-actin cDNA of each sample. The dotted line represents the mean of NF samples.