Summary of RIT-seq outputs following RNAi library selection in NHS.
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aAnnotations derived from GeneDB, http://www.genedb.org/Homepage/Tbruceibrucei927.b‘Tagged’ reads incorporate a 14-bp RNAi construct signature.cPlotted in Figure 2A.dHigh-confidence ‘hits’ characterised by >99 reads containing the 14-base RNAi construct signature, and >1 distinct RNAi target fragment.eBloodstream-form (BSF) T. b. brucei RNAi library fitness ratio derived from reference [24]; six-day induced read count divided by uninduced read count.fOther ‘hits’ are characterised by massive gain-of-fitness during RNAi induction (Tb927.8.6870) [24], a single RNAi target sequence (Tb927.5.1540, Tb927.6.2930), or
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2015-12-02
