mRNAseq of murine PancOVA tumor cells after coculture with murine qPSCs, myCAFs or iCAFs

To assess the influence of various fibroblast populations on the tumor cells in the PDAC microenvironment in Vitro,a co-culture of murine PancOVA tumor cells and quiescent (qPSC) or activated and primed inflammatory (iCAF) or myofibroblastic (myCAF) Fibroblasts was established, FACS sorted into fibroblast and tumor cell fractions after 48h and mRNA sequencing of PancOVA tumor cells from this coculture was performed Fibroblasts and PancOVA cells were seeded as co-cultures for 48h in matrigel droplets. All conditions (iCAF, myCAF, qPSC) were done in triplicates, non-activated (qPSC) fibroblasts were used as background expression control.