HSFA1a controls plant heat stress response through its action on the 3D chromatin reorganization of enhancer-promoter interactions [ATAC-seq]

We investigated the role of HSFA1a, a master regulator of heat stress response, in this reorganization through promotion of the formation of promoter/enhancer chromatin loops. We performed ATAC-seq experiments which provide accessible chromatin sites that can be used to identify putative regulatory elements coming in physical contact with promoters when they are actived. Overall design: Comparative chromatin accessibility patterns analysis of ATAC-seq data for tomato M82 wild type after 0h, 1h and 6h of heat stress. 2 biological replicates for each time point.