Long-term effects of Elastase inhibition on human islets at single-cell resolution

To evaluate the long-term effect of elastase inhibition using telaprevir, sivelestat, or DMSO on human islet cells. Overall design: Human islets (N=3) were treated for 24 hours with DMSO, telaprevir (1 and 100 µg/ml), or sivelestat (100 µg/ml) to evaluate the changes in gene expression levels in endocrine and exocrine cells using single-cell RNA-seq (scRNA-seq) procedures.