Introduction: Genome editing by CRISPR-Cas9 approaches offers promise for introducing or correcting disease-associated mutations for research and clinical applications. Nonhuman primates are physiolog
Purpose: Intermuscular bones (IBs) are hard spicules, mainly existing in the myosepta of recent vertebrates. This evolutionary trait has puzzled biologists and consumers, and the molecular basis of IB
To improve the efficiency of homology-directed repair of double-stranded breaks introduced by CRISPR-Cas9, a Cas9-PRDM9 (PR/SET Domain 9) fusion protein was generated and tested in HEK-293T cells. RNA
